More tools for our toolkit: The application of HEL-299 cells and dsRNA-nanoparticles to study human coronaviruses in vitroArticle Published on 2022-11-012022-11-15 Journal: Virus research [Category] SARS, 치료법, 치료제, [키워드] ACE2 receptor Analysis Antiviral antiviral genes antiviral response antiviral therapy can be used Cell cell line cells Combination competent Complete coronavirus Coronavirus replication CXCL10 cytidylic acid double-stranded expand fibroblast Gene Expression HCoV-229E HCoV-NL63 help Human human coronavirus human coronaviruses human lung IFN IFNs IFNβ in vitro increase in inducer Infection inhibit innate immune interferons ISG15 limit MERS-CoV Nanoparticles Pathogens Poly I:C. post infection PROTECT provided required SARS-CoV-2 SARS-CoV-2 pandemic stimulate translated Treatment Type I IFN type I interferon virus production were infected while [DOI] 10.1016/j.virusres.2022.198925 PMC 바로가기
Divergent SARS-CoV-2-specific T cell responses in intensive care unit workers following mRNA COVID-19 vaccinationArticle Published on 2022-10-062022-11-15 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 진단, [키워드] acute respiratory syndrome analyzed antibody CD4 CD4+ CD4+ T cells CD8 CD8+ CD8+ T cells Cellular immune response competent coronavirus COVID-19 COVID-19 vaccination develop dose elicited ELISPOT exhibited had no healthy individuals humoral ICU immune protection immune response individual individuals information intensive care intensive care unit workers lack maintain mRNA mRNA COVID-19 vaccines mRNA vaccine neutralizing antibody proportion Protein protein S reactive receiving reduction in SARS-CoV-2 SARS-CoV-2-specific T cell Seroconversion seronegative Specific T-cell response susceptibility T cell T cell response T cells T lymphocyte T-cell the S protein the SARS-CoV-2 the spike protein vaccination vaccination. viral infection Viral protein [DOI] 10.3389/fimmu.2022.942192 PMC 바로가기
Cell-Based Platform for Antigen Testing and Its Application for SARS-CoV-2 InfectionArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료제, [키워드] active infection acute respiratory syndrome amplification Antigen antigen test applicability application asymptomatic individual Calu-3 can be used CAR CAR T-cell Care Cell cell line cell-based sensors characterized Community competent conducted control sample coronavirus COVID-19 COVID-19 diagnostics detect diagnosing effective facilitate function Health host cell implementation individual infected with SARS-CoV-2 Infection infections lack mice molecular mRNA offer oropharyngeal swab pandemic Pandemics PCR platform Preclinical present antigen qPCR quantitative PCR reporter reporter cell. robust SARS-CoV-2 Signaling specificity spike glycoprotein symptomatic target pathogen tested Testing viruses Volume [DOI] 10.1128/spectrum.00731-22 PMC 바로가기
Strong T-cell activation in response to COVID-19 vaccination in multiple sclerosis patients receiving B-cell depleting therapiesB 세포 고갈 요법을받는 다발성 경화증 환자에서 COVID-19 예방 접종에 대한 강력한 T 세포 활성화Article Published on 2022-08-052022-09-11 Journal: Frontiers in Immunology [Category] SARS, 바이오마커, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adaptive Adaptive immune response Adaptive immunity analyzed anti-CD20 anti-CD20 therapy antibodies antibody production B-cell binding blocking activity BNT162b2 Cellular immune response cellular response Cohort competent Complication condition controls coronavirus COVID-19 COVID-19 vaccination depleting disease driven by form forms hACE2 healthy control humoral Humoral and cellular responses IFNγ IgG antibodies IgG antibody IL-2 immunocompromised individuals Immunotherapy implication individual individuals Infection investigation mRNA mRNA-1273 mRNA-based mRNA-based vaccine multiple sclerosis no difference Ocrelizumab Patient patients proliferation RBD receiving Receptor binding domain recognizing reduced regimen relapsing respiratory rituximab SARS-CoV-2 Seroconversion seroconversion rate severe acute respiratory syndrome Coronavirus significantly significantly higher Spike protein Strong strong T-cell susceptible T-cell T-cell activation T-cell Response therapy vaccination vaccination. Vaccine design virus infection [DOI] 10.3389/fimmu.2022.926318 PMC 바로가기 [Article Type] Article
Simulated RBC antibody identification training panels created using SARS-CoV-2 kodecytes and immune plasmaSARS-CoV-2 kodecytes 및 면역 혈장을 사용하여 만든 시뮬레이션된 RBC 항체 식별 교육 패널Article Published on 2022-08-012022-09-12 Journal: Transfusion [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Accuracy antibody antibody screening autoantibody can be used Cell competent develop Donor evaluated feasibility Frequency gel IgG IgG antibody immune Kodecyte maintain manual peptide plasma plasma sample platform positive Practical training Precision profile RBC Reactions required SARS-CoV-2 SARS-CoV-2 antibodies technology tested training unmodified cell warm [DOI] 10.1111/trf.17004 PMC 바로가기 [Article Type] Article
BSL2-compliant lethal mouse model of SARS-CoV-2 and variants of concern to evaluate therapeutics targeting the Spike proteinSARS-CoV-2의 BSL2 호환 치명적인 마우스 모델 및 Spike 단백질을 표적으로 하는 치료제 평가를 위한 우려 변종Article Published on 2022-07-282022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 transgenic mice acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 animal model antibody both virus both viruses brains BSL-2 BSL-2 mouse model BSL2 BSL3 BST2 can be used CCL5 change competent concern conditions coronavirus COVID-19 COVID19 CXCL10 disease Disease progression drugs Encephalitis evaluate experimental therapeutics facilitate faster generate GFP glycoprotein hACE2 host-pathogen interactions in vivo increased expression Infection interferon IRF7 K18-hACE2 K18-hACE2 transgenic mice lethal infection LILRB4 lung Lungs mass vaccination mice modulate mouse model mutations Neonatal neuronal infection neurotropism pathogenicity prophylactic treatment Protein pseudotyped Pseudotyped virus Receptor binding domain replaced REPLACED BY Replication reported SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variants SARS-CoV-2 viruses SARS2 severe acute respiratory syndrome Coronavirus Spike protein spike protein. stomatitis successive wave successive waves the SARS-CoV-2 the Spike the spike protein therapeutic Therapeutics transgenic transgenic mice Transmission vaccination Vaccine development variant variants of concern vesicular vesicular stomatitis vesicular stomatitis virus virulence virus virus VoC VSV VSV (vesicular stomatitis virus) [DOI] 10.3389/fimmu.2022.919815 PMC 바로가기 [Article Type] Article
Single Immunization with Recombinant ACAM2000 Vaccinia Viruses Expressing the Spike and the Nucleocapsid Proteins Protects Hamsters against SARS-CoV-2-Caused Clinical Disease스파이크 및 뉴클레오캡시드 단백질을 발현하는 재조합 ACAM2000 백시니아 바이러스를 사용한 단일 면역은 SARS-CoV-2 유발 임상 질병으로부터 햄스터를 보호합니다Article Published on 2022-05-112022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법, [키워드] ACAM2000 Antibody titer Antigen antigens Breakthrough infection candidate clinical Combination competent Continuous COVID-19 COVID-19 vaccine COVID-19 vaccines deleted demonstrated described develop E3L Efficacy ENhance expected expressed expressing hamster hamsters highlight host range human cells immune responses Immunity immunization immunogenic Increasing induce Infection initial innate immune responses intramuscular investigated K3L long-lasting lymphocyte N protein Neutralizing antibody titer neutrophil Neutrophil-to-lymphocyte ratio nucleocapsid nucleocapsid antigen nucleocapsid antigens nucleocapsid protein Nucleocapsid proteins ortholog platform poxvirus Protective Protein recombinant recombinant COVID-19 vaccine Recovery time reduced reduced viral loads replaced SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant SARS-CoV-2 variants shorter recovery time shown significantly spike spike and nucleocapsid proteins the SARS-CoV-2 the Spike the vaccine Vaccine vaccine candidate vaccinia vaccinia virus vaccinia. variants Viral virus virus replication weight loss were expressed [DOI] 10.1128/jvi.00389-22 PMC 바로가기 [Article Type] Article
Discovery and Use of Long dsRNA Mediated RNA Interference to Stimulate Antiviral Protection in Interferon Competent Mammalian Cells인터페론 유능한 포유동물 세포에서 항바이러스 보호를 자극하기 위한 긴 dsRNA 매개 RNA 간섭의 발견 및 사용Article Published on 2022-05-062022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] Antiviral Antiviral effect antiviral response antiviral responses cell line cells cellular cleaved comparable competent complementary Concentration cornerstone CoV dependent on Dicer dsRNA Evidence explanation expressing fluorescent functional gene targeting generate green fluorescent protein HCoV HCoV-229E human coronavirus human coronaviruses IFN immune defense induce inducer inhibit Interference interferons knockdown Lineage Long long dsRNA mammalian mammalian cell mammalian cells Mediated mRNA pathway platform preventing infection produced protection Protein provide required responsible retained RISC RNA RNA interference RNA-induced silencing complex RNAi RNAi pathway SARS-CoV-2 replication sequence shown significantly siRNA siRNAs stimulate stomatitis suppress therapeutic These cells threshold type I interferon vesicular stomatitis vesicular stomatitis virus viral inhibition. virus VSV-GFP [DOI] 10.3389/fimmu.2022.859749 PMC 바로가기 [Article Type] Article
Expression analysis of IFNAR1 and TYK2 transcripts in COVID-19 patientsCOVID-19 환자에서 IFNAR1 및 TYK2 전사체의 발현 분석Article Published on 2022-05-012022-09-11 Journal: Cytokine [Category] COVID19(2023년), SARS, 바이오마커, 유전자 메커니즘, 진단, [키워드] Analysis Biomarker blood sample Cell competent Control controls COVID-19 COVID-19 case COVID-19 cases COVID-19 patient COVID-19 patients disease severity down-regulated down-regulation expression female ICU admission IFN response IFNAR1 immune responses Incapacity Jak kinases male member molecular mechanism not different p value Patient Protein Regulation required SARS-CoV-2 Signaling significantly transcript TYK2 TYK2. Type I IFN tyrosine venous venous blood [DOI] 10.1016/j.cyto.2022.155849 PMC 바로가기 [Article Type] Article
Main protease mutants of SARS-CoV-2 variants remain susceptible to nirmatrelvirSARS-CoV-2 변이체의 주요 프로테아제 돌연변이체는 여전히 니르마트렐비르에 감수성입니다Article Published on 2022-04-152022-09-11 Journal: Bioorganic & medicinal chemistry letters [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] anticipated antiviral agent Antiviral agents B.1.1.529 B.1.351 Beta catalytically competent Coronaviral COVID-19 COVID-19 pandemic drug target Effectiveness Efficacy expressed in vitro data inhibitor inhibitors Lambda M pro main protease Missense mutation Multiple multiple mutations mutant Mutation Nirmatrelvir not compromised omicron P.2 PF-07321332 prevalent protease public health threat SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 variant Specific Spike protein susceptible the spike protein Vaccines variant variants variants of SARS-CoV-2 variants. wildtype [DOI] 10.1016/j.bmcl.2022.128629 PMC 바로가기 [Article Type] Article